Anticandidal and antioxidant potencies of endophytic fungi associated with Tinospora crispa

Abstract. Fathoni A, Hudiyono S, Cahyana AH, Ilyas M, Purnaningsih I, Agusta A. 2023. Anticandidal and antioxidant potencies of endophytic fungi associated with Tinospora crispa. Biodiversitas 24: 2547-2555. Natural products including fungi, have opportunities for medicinal uses as anticandidal agents. This study aimed to investigate the activities isolated from Brotowali (Tinospora crispa (L.) Miers ex Hook.fil. & Thomson) using disc diffusion, thin layer chromatography (TLC) dot-blot, microdilution methods. Eighty extracts were screened activity against Candida albicans (C.P.Robin) Berkhout antioxidants 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. Disc diffusion results showed that 38 had an inhibition zone diameter (IZD) 7.00±00 18.1± 0.29 mm 4 minimum inhibitory concentration (MIC) values 32-64 µg/mL (strong, MIC<100). The TLC dot-blot 23 8 very strong 50% (IC50) 7.76±0.77 25.54±1.37 µg/mL, while index (AAI) ranged 2.01±0.10 6.64±0.63 (very strong, AAI>2). Results Pearson correlations coefficient between total phenolic content (TPC) value AAI value, revealed there was a low correlation, correlation IZD MIC has moderate negative (the r 0.332, -0.673, respectively (P<0.01). Purification bioactive compounds is necessary, T. can serve potential source natural